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pe cy7 pe vio 770 cd161 miltenyi 191b8  (Miltenyi Biotec)


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    Miltenyi Biotec pe cy7 pe vio 770 cd161 miltenyi 191b8
    Pe Cy7 Pe Vio 770 Cd161 Miltenyi 191b8, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe cy7 pe vio 770 cd161 miltenyi 191b8/product/Miltenyi Biotec
    Average 93 stars, based on 116 article reviews
    pe cy7 pe vio 770 cd161 miltenyi 191b8 - by Bioz Stars, 2026-04
    93/100 stars

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    Image Search Results


    Journal: Immunity

    Article Title: Differential IRF8 Transcription Factor Requirement Defines Two Pathways of Dendritic Cell Development in Humans

    doi: 10.1016/j.immuni.2020.07.003

    Figure Lengend Snippet:

    Article Snippet: Mouse anti-human CD161 PE-Cy7, clone HP-3G10 , Thermo Fisher Scientific , Cat# 25-1619-42; RRID: AB_10807086.

    Techniques: Purification, Recombinant, Concentration Assay, Saline, Staining, Antibody Labeling, RNA Sequencing, Software, Sterility

    CCR6 + CD4 + Th cells in the cerebrospinal fluid predominantly secrete IFNγ, not IL-17A, and are elevated in MS. A. Representative data demonstrating CCR6 expression on IL-17 + and IFNγ + cells (gated on CD3 + CD45RO + CD8 − cells) in PBMC and matched CSF cells. Numbers represent the percentage of cells within the quadrant, with negative gates set based on an un-stimulated controls. B, C. The percentage of CCR6 + CD4 + T cells that expresses either IL-17A (B) or IFNγ (C) in PBMC and matched CSF. D-F. The percentage of CD4 + memory T cells of a CCR6 + IL-17A + (D), CCR6 + IFNγ + (E) or CCR6 − IFNγ + phenotype (F). G-I. The absolute number of CCR6 + IL-17A + (G), CCR6 + IFNγ + (H) or CCR6 − IFNγ + (I) CSF CD4 + memory T cells. Box and whiskers plots are shown with minimum and maximum values. Wilcoxon matched-pairs signed rank (B-F) and Mann-Whitney tests (G–I) ( * = p < 0.05, ** = p < 0.01, *** = p < 0.001); all other comparisons were non-significant (p > 0.05).

    Journal: Brain, Behavior, and Immunity

    Article Title: CCR6 + Th cells in the cerebrospinal fluid of persons with multiple sclerosis are dominated by pathogenic non-classic Th1 cells and GM-CSF-only-secreting Th cells

    doi: 10.1016/j.bbi.2017.03.008

    Figure Lengend Snippet: CCR6 + CD4 + Th cells in the cerebrospinal fluid predominantly secrete IFNγ, not IL-17A, and are elevated in MS. A. Representative data demonstrating CCR6 expression on IL-17 + and IFNγ + cells (gated on CD3 + CD45RO + CD8 − cells) in PBMC and matched CSF cells. Numbers represent the percentage of cells within the quadrant, with negative gates set based on an un-stimulated controls. B, C. The percentage of CCR6 + CD4 + T cells that expresses either IL-17A (B) or IFNγ (C) in PBMC and matched CSF. D-F. The percentage of CD4 + memory T cells of a CCR6 + IL-17A + (D), CCR6 + IFNγ + (E) or CCR6 − IFNγ + phenotype (F). G-I. The absolute number of CCR6 + IL-17A + (G), CCR6 + IFNγ + (H) or CCR6 − IFNγ + (I) CSF CD4 + memory T cells. Box and whiskers plots are shown with minimum and maximum values. Wilcoxon matched-pairs signed rank (B-F) and Mann-Whitney tests (G–I) ( * = p < 0.05, ** = p < 0.01, *** = p < 0.001); all other comparisons were non-significant (p > 0.05).

    Article Snippet: The following antibodies were used in this study; anti-CD3 APC-eFluor 780 (UCHT1), -CD4 APC (OKT4), -CD161 PE-Cy7 (HP-3G10), -IFNγ eFluor450 or PE (45.B3), -IL-17A eFluor 488 (eBio64DEC17), -IL-22 PE (22URT1), -IL-21 PE (eBio3A3-N2), -IL-17F PE (SHLR17), -T-bet PE (eBio4B10), -RORγt PE (AFKJS-9) (eBioscience), anti-CD3 Brilliant Violet 510 (OKT 3), -CD4 PE-Cy7 (OKT4), -CD8 Brilliant Violet 510 (RPA-T8), -CD25 PerCP-Cy5.5 (BC96), -CCR6 PE or Alexa Fluor (AF) 647 (G034E3), -CCR7 AF488 (G043H7), -CXCR3 AF647 (G025H7), anti-CD45RA PE-CF594 (HI100), -CD45RO PE-CF594 (UCHL1), -GM-CSF PE (BVD2-21C11) (BD Pharmingen), and anti-CD4 PE-Vio770 (VIT 4) (Miltenyi Biotec).

    Techniques: Expressing, MANN-WHITNEY

    CCR6 expressed on IFNγ + IL-17A − CD4 + memory T cells is functional. A. Expression of CXCR3 and CCR6 gated on CD4 + CD45RO + memory T cells. B, C. Median fluorescence intensity (MFI) of CCR6 (B) and CXCR3 (C) in CD4 + CD45RO + memory T cells expressing combinations of CCR6, IFNγ and IL-17A. Repeated measures ANOVA with Bonferroni's multiple comparison test (n = 5); * = p < 0.05, ** = p < 0.01, *** = p < 0.001. All other comparisons were non-significant (p > 0.05). D, E. Purified CD4 + T cells were migrated in a transwell in response to the indicated concentrations of CCL20 (D) or CXCL12 (E). The migrated and non-migrated fractions were then stimulated with PMA and ionomycin before determining IFNγ and IL-17 expression, allowing calculation of the migration of each subset. Data represent the mean and SEM of three independent experiments.

    Journal: Brain, Behavior, and Immunity

    Article Title: CCR6 + Th cells in the cerebrospinal fluid of persons with multiple sclerosis are dominated by pathogenic non-classic Th1 cells and GM-CSF-only-secreting Th cells

    doi: 10.1016/j.bbi.2017.03.008

    Figure Lengend Snippet: CCR6 expressed on IFNγ + IL-17A − CD4 + memory T cells is functional. A. Expression of CXCR3 and CCR6 gated on CD4 + CD45RO + memory T cells. B, C. Median fluorescence intensity (MFI) of CCR6 (B) and CXCR3 (C) in CD4 + CD45RO + memory T cells expressing combinations of CCR6, IFNγ and IL-17A. Repeated measures ANOVA with Bonferroni's multiple comparison test (n = 5); * = p < 0.05, ** = p < 0.01, *** = p < 0.001. All other comparisons were non-significant (p > 0.05). D, E. Purified CD4 + T cells were migrated in a transwell in response to the indicated concentrations of CCL20 (D) or CXCL12 (E). The migrated and non-migrated fractions were then stimulated with PMA and ionomycin before determining IFNγ and IL-17 expression, allowing calculation of the migration of each subset. Data represent the mean and SEM of three independent experiments.

    Article Snippet: The following antibodies were used in this study; anti-CD3 APC-eFluor 780 (UCHT1), -CD4 APC (OKT4), -CD161 PE-Cy7 (HP-3G10), -IFNγ eFluor450 or PE (45.B3), -IL-17A eFluor 488 (eBio64DEC17), -IL-22 PE (22URT1), -IL-21 PE (eBio3A3-N2), -IL-17F PE (SHLR17), -T-bet PE (eBio4B10), -RORγt PE (AFKJS-9) (eBioscience), anti-CD3 Brilliant Violet 510 (OKT 3), -CD4 PE-Cy7 (OKT4), -CD8 Brilliant Violet 510 (RPA-T8), -CD25 PerCP-Cy5.5 (BC96), -CCR6 PE or Alexa Fluor (AF) 647 (G034E3), -CCR7 AF488 (G043H7), -CXCR3 AF647 (G025H7), anti-CD45RA PE-CF594 (HI100), -CD45RO PE-CF594 (UCHL1), -GM-CSF PE (BVD2-21C11) (BD Pharmingen), and anti-CD4 PE-Vio770 (VIT 4) (Miltenyi Biotec).

    Techniques: Functional Assay, Expressing, Fluorescence, Comparison, Purification, Migration

    CCR6 + IFNγ + CD4 + memory T cells in the CSF secrete GM-CSF and are elevated in MS. Matched PBMC and CSF cells were stimulated with PMA and ionomycin and stained for surface CCR6, and intracellular IFNγ, IL-17 and GM-CSF. Representative data is shown from a person with MS (A). All samples were gated on CD3 + CD4 + CD45RO + memory T cells. Box and whiskers plots are shown with minimum and maximum values. Wilcoxon matched-pairs signed rank test (B,C); * = p < 0.05, ** = p < 0.01. All other comparisons were non-significant (p > 0.05).

    Journal: Brain, Behavior, and Immunity

    Article Title: CCR6 + Th cells in the cerebrospinal fluid of persons with multiple sclerosis are dominated by pathogenic non-classic Th1 cells and GM-CSF-only-secreting Th cells

    doi: 10.1016/j.bbi.2017.03.008

    Figure Lengend Snippet: CCR6 + IFNγ + CD4 + memory T cells in the CSF secrete GM-CSF and are elevated in MS. Matched PBMC and CSF cells were stimulated with PMA and ionomycin and stained for surface CCR6, and intracellular IFNγ, IL-17 and GM-CSF. Representative data is shown from a person with MS (A). All samples were gated on CD3 + CD4 + CD45RO + memory T cells. Box and whiskers plots are shown with minimum and maximum values. Wilcoxon matched-pairs signed rank test (B,C); * = p < 0.05, ** = p < 0.01. All other comparisons were non-significant (p > 0.05).

    Article Snippet: The following antibodies were used in this study; anti-CD3 APC-eFluor 780 (UCHT1), -CD4 APC (OKT4), -CD161 PE-Cy7 (HP-3G10), -IFNγ eFluor450 or PE (45.B3), -IL-17A eFluor 488 (eBio64DEC17), -IL-22 PE (22URT1), -IL-21 PE (eBio3A3-N2), -IL-17F PE (SHLR17), -T-bet PE (eBio4B10), -RORγt PE (AFKJS-9) (eBioscience), anti-CD3 Brilliant Violet 510 (OKT 3), -CD4 PE-Cy7 (OKT4), -CD8 Brilliant Violet 510 (RPA-T8), -CD25 PerCP-Cy5.5 (BC96), -CCR6 PE or Alexa Fluor (AF) 647 (G034E3), -CCR7 AF488 (G043H7), -CXCR3 AF647 (G025H7), anti-CD45RA PE-CF594 (HI100), -CD45RO PE-CF594 (UCHL1), -GM-CSF PE (BVD2-21C11) (BD Pharmingen), and anti-CD4 PE-Vio770 (VIT 4) (Miltenyi Biotec).

    Techniques: Staining

    CCR6 + CD4 + GM-CSF-only-secreting T cells are elevated in MS. The percentage (A–E) of CD4 + memory T cells expressing the indicated combinations of CCR6, IL-17A, IFNγ and GM-CSF in PBMC and matched CSF. The absolute numbers of CSF CD4 + memory T cells of each phenotype are shown in F-J. Box and whisker plots are shown with minimum and maximum values for each cohort. Wilcoxon matched-pairs signed rank (A-E) and Mann-Whitney tests (B–J) ( * = p < 0.05, ** = p < 0.01); all other comparisons were non-significant (p > 0.05).

    Journal: Brain, Behavior, and Immunity

    Article Title: CCR6 + Th cells in the cerebrospinal fluid of persons with multiple sclerosis are dominated by pathogenic non-classic Th1 cells and GM-CSF-only-secreting Th cells

    doi: 10.1016/j.bbi.2017.03.008

    Figure Lengend Snippet: CCR6 + CD4 + GM-CSF-only-secreting T cells are elevated in MS. The percentage (A–E) of CD4 + memory T cells expressing the indicated combinations of CCR6, IL-17A, IFNγ and GM-CSF in PBMC and matched CSF. The absolute numbers of CSF CD4 + memory T cells of each phenotype are shown in F-J. Box and whisker plots are shown with minimum and maximum values for each cohort. Wilcoxon matched-pairs signed rank (A-E) and Mann-Whitney tests (B–J) ( * = p < 0.05, ** = p < 0.01); all other comparisons were non-significant (p > 0.05).

    Article Snippet: The following antibodies were used in this study; anti-CD3 APC-eFluor 780 (UCHT1), -CD4 APC (OKT4), -CD161 PE-Cy7 (HP-3G10), -IFNγ eFluor450 or PE (45.B3), -IL-17A eFluor 488 (eBio64DEC17), -IL-22 PE (22URT1), -IL-21 PE (eBio3A3-N2), -IL-17F PE (SHLR17), -T-bet PE (eBio4B10), -RORγt PE (AFKJS-9) (eBioscience), anti-CD3 Brilliant Violet 510 (OKT 3), -CD4 PE-Cy7 (OKT4), -CD8 Brilliant Violet 510 (RPA-T8), -CD25 PerCP-Cy5.5 (BC96), -CCR6 PE or Alexa Fluor (AF) 647 (G034E3), -CCR7 AF488 (G043H7), -CXCR3 AF647 (G025H7), anti-CD45RA PE-CF594 (HI100), -CD45RO PE-CF594 (UCHL1), -GM-CSF PE (BVD2-21C11) (BD Pharmingen), and anti-CD4 PE-Vio770 (VIT 4) (Miltenyi Biotec).

    Techniques: Expressing, Whisker Assay, MANN-WHITNEY